High Ability of Bacillus Cereus Strain Isolated from Chromite Mine in Bioremediation of Carcinogenic Chromium-Contaminated Wastewater


Cr (VI) is a toxic metal produced as a by-product of numerous industrial processes. Thus, strategies employed to decontaminate environmental chromate focus on reducing it to the less toxic form. In the present study, a new low cost, easily available, and environmentally friendly method was used for removal of Cr (VI). Here we report the isolation of high chromate tolerant strain with MIC value of 75000 ppm in LB medium (pH 7) from chromium mine. Based on the 16S rRNA gene analysis, strain Ckcr-6 was identified as Bacillus sp. with 99% similarity and was referred to as Bacillus cereous. The (VI) removal efficiency was studied as a function of contact pH, bacterial dose, chromium concentration, temperature and stirring speed. Result showed that in all concentrations there was a reduction of approximately 95% of the Cr (VI) after 24 h incubation. After optimization this strains was shown to be of a high efficiency in detoxifying chromate, this could rapidly remove up to 100 ppm of Cr (VI) to a non-detectable level over 3h.


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